Thermo Fisher Scientific Phospho-CDC25C (Ser216) Polyclonal Antibody
CDC25C 단백질의 Ser216 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. 인간 및 생쥐 반응성. 고순도 정제, PBS/glycerol buffer에 보존. 연구용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC25C (Accession P30307), corresponding to amino acid residues around phosphorylated Ser216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816006 |
Product Specific Information
This antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.
Target Information
M-phase inducer phosphatase 3 is an enzyme encoded by the CDC25C gene in humans. This gene is highly conserved and plays a critical role in regulating cell division. The encoded protein is a tyrosine phosphatase belonging to the Cdc25 family, responsible for dephosphorylating cyclin B-bound CDC2 (CDK1) to trigger mitosis. It may also suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants have been described, though many are not fully characterized.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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