Thermo Fisher Scientific Insulin Monoclonal Antibody (5A6A4)

Applications

ELISA

• Tested Dilution: 0.05–0.2 µg/mL

Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids, and inhibits the breakdown of glycogen, protein, and fat.

Proinsulin is the prohormone precursor to insulin made in the pancreas. It is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence; the two other ends (alpha and beta chains) remain connected by disulfide bonds.

Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

GenScript Insulin Antibody (5A6A4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant Insulin expressed in yeast.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein, and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids.

Insulin is a 51-amino acid polypeptide produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide.

Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.