Thermo Fisher Scientific beta-tubulin Monoclonal Antibody (2G7D4)
Beta-tubulin 단백질을 표적으로 하는 Mouse monoclonal antibody (Clone 2G7D4). Western blot, IHC, Flow cytometry 등 다양한 응용 가능. 미세소관 구조 연구 및 로딩 컨트롤용으로 적합. Lyophilized 형태로 안정적 보관 가능.
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- A0171740
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.2–1 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10–20 µg/mL | - |
| Flow Cytometry (Flow) | 1 µg / 1 × 10⁶ cells | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Fish, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G7D4 |
| Immunogen | Purified human recombinant full-length beta-tubulin protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. It is a dimer of alpha (α) and beta (β) chains. β-tubulin is ubiquitously expressed with highest levels in spleen, thymus, and immature brain, and has a highly acidic C-terminal region which may bind cations such as calcium.
This antibody can be used as a loading control on Western blots. The GenScript β-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length β-tubulin protein.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance.
Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, although the function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, and Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin dimers (alpha and beta subunits, ~55 kDa each). They play key roles in chromosome segregation, intracellular transport, ciliary and flagellar movement, and structural support of the cytoskeleton.
Because beta-tubulin is ubiquitously expressed, it is frequently used as a loading control for protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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