Thermo Fisher Scientific Akirin2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 8 and 203 of Akirin 2 (Uniprot ID#Q53H80) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKIRIN2,uniProtId:Q53H80-1,ncbiNodeId:9606,antigenRange:8-203,antigenLength:203,antigenImageFileName:PA5-34875_AKIRIN2_Q53H80-1_Rabbit.svg,antigenImageFileNamePDP:PA5-34875_AKIRIN2_Q53H80-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552227

Product Specific Information

Recommended positive controls: A549, HeLa, HCT116.

Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (81%), Xenopus laevis (83%), Pig (98%), Chicken (94%), Sheep (97%), Chimpanzee (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-kappa-B-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-kappa-B pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-a and IL-1-b signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.