Thermo Fisher Scientific PARC/H7-AP1 Polyclonal Antibody
PARC/H7-AP1 폴리클로날 항체로 인간 단백질에 반응하며, Western blot 및 Immunoprecipitation에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체로, 1 mg/mL 농도. p53 관련 단백질 복합체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 900 and 950 of human P53-associated parkin-like cytoplasmic protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase, has been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression promotes cytoplasmic sequestration of p53. PARC knockout mice were viable and exhibited no obvious phenotype, suggesting functional redundancy with other E3 ubiquitin ligases such as the cullin family. p53 binding to PARC may serve to regulate PARC function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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