Thermo Fisher Scientific PARC/H7-AP1 Polyclonal Antibody
PARC/H7-AP1 폴리클로날 항체로 인간 단백질 검출에 적합. Western blot 및 Immunoprecipitation에 사용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체. 4°C에서 보관하며 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:30,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2475 and the C-terminus (residue 2527) of human P53-associated parkin-like cytoplasmic protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The continued localization of p53 to the nucleus is essential for its tumor suppressor function. PARC, a large Parkin-like ubiquitin ligase, acts as a cytoplasmic anchor protein in p53-associated complexes. Inactivation of PARC results in nuclear localization of p53 and activation of p53-dependent apoptosis, whereas overexpression promotes cytoplasmic sequestration of p53. PARC knockout mice remain viable without obvious phenotype, suggesting redundancy with other E3 ubiquitin ligases such as cullin family proteins. p53 binding to PARC may also regulate PARC function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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