Thermo Fisher Scientific CD1d Monoclonal Antibody (51.1), eBioscience
인간 CD1d 단백질을 인식하는 51.1 클론의 마우스 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 응용에 적합합니다. 비결합형 형식이며, 0.5 mg/mL 농도의 액상 제품입니다. CD1d–NKT 세포 상호작용 차단 기능이 보고되었습니다.
- 카탈로그번호
- 14-0016-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD1d Monoclonal Antibody (51.1), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 5 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | 2 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 51.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864802 |
Additional Formats
Product Specific Information
The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family with similarity to non-polymorphic MHC Class I-like molecules. CD1d is a conserved transmembrane receptor of the Immunoglobulin Superfamily, capable of associating with beta-2 microglobulin or existing independently as a nonglycosylated protein. This dual expression suggests different mechanisms for presenting glycolipid molecules to CD1d-reactive NKT cells.
CD1d is expressed on peripheral B cells, resting monocytes, cortical thymocytes, and polarized on intestinal epithelial cells. Low-level intracellular expression is observed in hepatocytes, and elevated expression is reported in HCV-infected livers.
The 51.1 monoclonal antibody has functional activity, capable of blocking interactions between CD1d-transfected cells and NKT cells.
Applications Reported: Flow cytometry, immunoprecipitation, and immunohistology of frozen tissue sections.
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.5 µg/test (in 100 µL final volume). Cell count: 10⁵–10⁸ cells/test. Optimize by titration for best performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD1d is a heavy chain associated with beta-2 microglobulin on cortical thymocytes, and can also be expressed independently on intestinal epithelial cells. It is the sole group 2 member of the CD1 family, encoding a transmembrane glycoprotein structurally related to MHC proteins that forms heterodimers with beta-2-microglobulin.
CD1 proteins mediate presentation of lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes located on chromosome 1, differing in localization and lipid ligand specificity. CD1d localizes to late endosomes and lysosomes through a tyrosine-based motif in its cytoplasmic tail.
Associated Diseases: Mycobacterium malmoense infection, autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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