Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), eBioscience
마우스 CD1d 단백질을 인식하는 1B1 클론의 Rat IgG2b 단일클론 항체로, Flow Cytometry, IHC, IP 등 다양한 응용에 사용 가능. 비접합형으로 제공되며, 고순도(>90%)로 정제. PBS 버퍼(0.09% sodium azide) 용액 형태로 4°C 보관.
- 카탈로그번호
- 14-0011-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 1 µg/test | 5 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 1 publication |
| Functional Assay | Assay-Dependent | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467036 |
Available Conjugated Formats
- Alexa Fluor 488
- APC
- Biotin
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available upon request
Product Specific Information
The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2-microglobulin but is functionally similar to MHC Class II. The 1B1 antibody detects CD1d at varying levels on mouse leukocytes and recognizes beta2m-associated CD1d.
Applications Reported: Flow cytometry, immunoprecipitation, and immunohistochemistry. Also used in in vivo and in vitro studies. For functional assays, use Functional Grade purified 1B1 (Product #16-0011).
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Optimize antibody titration for best performance.
Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD1d is a heavy chain associated with beta-2 microglobulin on cortical thymocytes. It is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a transmembrane glycoprotein structurally related to MHC proteins, forming heterodimers with beta-2-microglobulin. CD1 proteins present lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes on chromosome 1, differing in localization and lipid ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.