Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available upon request

Product Specific Information

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2-microglobulin but is functionally similar to MHC Class II. The 1B1 antibody detects CD1d at varying levels on mouse leukocytes and recognizes beta2m-associated CD1d.

Applications Reported: Flow cytometry, immunoprecipitation, and immunohistochemistry. Also used in in vivo and in vitro studies. For functional assays, use Functional Grade purified 1B1 (Product #16-0011).

Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Optimize antibody titration for best performance.

Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD1d is a heavy chain associated with beta-2 microglobulin on cortical thymocytes. It is the sole group 2 member of the CD1 family of MHC-like glycoproteins. The CD1d gene encodes a transmembrane glycoprotein structurally related to MHC proteins, forming heterodimers with beta-2-microglobulin. CD1 proteins present lipid and glycolipid antigens to T cells. The human genome contains five CD1 family genes on chromosome 1, differing in localization and lipid ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.