Thermo Fisher Scientific DUT Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000–1:2,000

Product Specifications

Product Specific Information

• Suggested positive control: antigen standard for DUT (transient overexpression lysate), HeLa whole cell extract, HeLa.

Target Information

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate.
This reaction serves two cellular purposes:

1. Providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication.
2. Limiting intracellular pools of dUTP.

Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.
Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.