Thermo Fisher Scientific alpha Dystroglycan Polyclonal Antibody
Human 반응성의 alpha Dystroglycan 폴리클로날 항체로 Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 합성 펩타이드 항원을 사용해 제작되었으며, 0.5 mg/mL 농도의 액상 형태로 제공. 근육 단백질 복합체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 5 µg/mL |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (HVGKHEYFMHATDK) corresponding to the internal amino acids of DAG1 (aa 569–582) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10984757 |
Product Specific Information
- Predicted to react with bovine and canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
Dystroglycan is a laminin-binding component of the dystrophin–glycoprotein complex, providing a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix.
Dystroglycan 1 is a candidate gene for mutations in autosomal recessive muscular dystrophies.
Reduction of dystroglycan 1 in Duchenne muscular dystrophy disrupts sarcolemma–matrix linkage, increasing muscle fiber susceptibility to necrosis.
It also acts as a dual receptor for agrin and laminin-2 in Schwann cell membranes.
Muscle and nonmuscle isoforms differ by carbohydrate composition, not protein sequence.
Alternative splicing produces multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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