Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), APC, eBioscience
Mouse PDCA-1(BST2, CD317) 표지에 특이적인 eBio927 단클론 항체로, APC 형광체가 결합된 Flow Cytometry용 항체. Plasmacytoid dendritic cell 식별에 적합하며 0.25 µg/test로 사용. 633–647 nm Excitation, 660 nm Emission.
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- 17-3172-8x (2개 옵션)
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Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), APC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Publications: View 12 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio927 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596503 |
Additional Formats
- Alexa Fluor 488
- Biotin
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse.
Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse (<0.5% of splenocytes) and is upregulated following IFN response stimulation.
PDCA-1 cycles between cell surface and intracellular compartments and may regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.
The eBio927 monoclonal antibody has functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify, and identify pDCs.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.25 µg/test (100 µL volume). Cell number range: 10⁵–10⁸ cells/test. Optimize by titration.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein involved in B-cell development and growth.
Surface BST2 expression on fibroblast cell lines facilitates stromal cell-dependent growth of pre-B-cell line DW34.
BST2 is highly expressed in B-cell development stages, T-cells, monocytes, NK cells, DCs, and myeloma cells.
It functions as an inhibitor of retrovirus release from human cells, antagonized by HIV-1 protein Vpu.
BST2 retention of virions on cell surfaces and endocytosis may represent a therapeutic target for HIV/AIDS.
BST2 dysfunction is also associated with stomatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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