Thermo Fisher Scientific ZBTB4 Monoclonal Antibody (OTI1F6), TrueMAB
ZBTB4 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC(P) 실험에 적합. Human, Mouse, Rat 반응성. Lyophilized 형태로 제공되며 PBS(8% trehalose) buffer에 보관. p53 활성화 관련 세포 반응 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 788–1013 of human ZBTB4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The ZBTB family comprises diverse zinc finger proteins containing a BTB (BR-C, ttk, and bab) domain.
Similar to Kaiso, ZBTB4 binds methylated DNA and represses transcription.
ZBTB4 associates with the Sin3/histone deacetylase co-repressor complex and represses expression of P21CIP1 as part of a heterodimeric complex with Miz1.
Depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis via regulation of P21CIP1, indicating ZBTB4 as a key determinant of the cellular p53 response.
HIPK2 phosphorylates and down-regulates ZBTB4 under normal conditions, with degradation increasing upon DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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