Thermo Fisher Scientific Catalase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

1:100,000-1:1,000,000

-

Immunoprecipitation (IP)

1:5,000-1:20,000

-

Product Specifications

Species Reactivity

Bovine

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

85 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but has not been specifically determined.

Target Information

Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara`s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.