Thermo Fisher Scientific Deoxyribonuclease I Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid.
• Dilute only prior to immediate use.
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum and purified Deoxyribonuclease I (Bovine Pancreas).
• Cross-reactivity against DNase I from other tissues and species may occur but has not been specifically determined.

Target Information

Internucleosomal DNA fragmentation following activation of endonucleases is a common endpoint of apoptosis.
DNase I, a Ca²⁺/Mg²⁺-dependent endonuclease expressed in mammalian tissues, mediates internucleosomal DNA degradation during apoptosis.
DNase I is highly polymorphic with at least six known alleles.
DNase II, an acidic deoxyribonuclease, acts downstream of caspase activation and also induces DNA digestion during apoptosis.
DNase I cleaves DNA to 5′-phosphodinucleotide and 5′-phosphooligonucleotide end-products, whereas DNase II produces 3′-phosphomononucleotide and 3′-phosphooligonucleotide end-products.
DNase II is typically cytoplasmic in epithelial cells but concentrated in nuclei of lens fibers, while DNase I is concentrated in nuclei of epithelial and fiber cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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