Thermo Fisher Scientific PHGDH Monoclonal Antibody (OTI4A1), TrueMAB
Human PHGDH 단백질을 인식하는 단클론 항체로 Western blot에 최적화됨. HEK293T 세포에서 생산된 full-length recombinant immunogen 사용. Lyophilized 형태로 제공되며 PBS buffer에 trehalose 포함. 연구용으로 L-serine 합성 관련 PHGDH 분석에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A1 |
| Immunogen | Full length human recombinant protein of human PHGDH produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PHGDH gene encodes an enzyme involved in the early steps of L-serine synthesis in animal cells.
L-serine is required for D-serine and other amino acid synthesis.
The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity.
Mutations in this gene have been associated with congenital microcephaly, psychomotor retardation, and other symptoms.
Multiple alternatively spliced transcript variants exist, though most full-length forms are not yet defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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