Thermo Fisher Scientific TRAF3 Polyclonal Antibody
TRAF3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 Immunocytochemistry에 적합하며, 인간·마우스·랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로 안정적 저장 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide from near the amino terminus of human TRAF3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at −20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151955 |
Product Specific Information
- Suggested positive control: HeLa cell lysate
- PA5-20166 can be used with blocking peptide PEP-0283
Target Information
Tumor necrosis factor (TNF)-induced signaling is mediated through association of TNF receptors (TNFR) with adaptor proteins such as TNF receptor-associated factors (TRAFs). TRAFs are cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g., RANK, CD30, CD40) and the interleukin-1 receptor.
The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Molecular cloning studies have identified six TRAFs (TRAF1–TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region of similarity to TNF-alpha receptor-associated factors. TRAF3 binds only a single site containing the sequence PEQET, whereas TRAF1 and TRAF2 can bind both PEQET and an additional downstream domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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