Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), NovaFluor Yellow 570, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–711)
• eFluor series (450–660)
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• NovaFluor Blue series
• Functional Grade
• Custom conjugation available upon request

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells and precipitates a 150, 90 kDa heterodimer.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
The N418 antibody is reported for use in flow cytometric analysis. Internal testing shows that NovaFluor Yellow 570 may non-specifically stain B cells in SJL mice, but not in BALB/c or C57BL/6 strains. Other strains have not been tested.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.4 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.

Blocking Buffer Recommendation:
This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
Add 5 µL per sample to antibody cocktails before combining with cells.
For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ technology utilizing nucleic acid dye structures, NovaFluor conjugates provide engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 568 nm; Emission: 568 nm; Laser: 561 nm (Yellow).

Target Information

CD11, along with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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