Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), NovaFluor Blue 660-40S, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), NovaFluor Blue 660-40S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.4 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Custom conjugation available upon request

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX, which associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, subsets of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis of mouse splenocytes
Applications Tested: ≤0.4 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be added (5 µL/sample) for optimal staining.
• Add buffer to antibody cocktails before combining with cells.

NovaFluor conjugates utilize Phiton™ technology based on engineered nucleic acid dye structures, offering optimized fluorescent signatures with reduced spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD11, together with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Found in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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