Thermo Fisher Scientific GABRA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein from the cytoplasmic loop of the alpha 1 subunit of rat GABRA1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.15 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559218

Product Specific Information

This antibody is predicted to react with bovine, canine, human and non-human primate based on 100% sequence homology.

This antibody is specific for the ~51 kDa alpha1-subunit of the GABAA Receptor in Western blots of rat brain.

Target Information

Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.