Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), PE, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor series, FITC, Functional Grade, NovaFluor series, PE-Cyanine5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright series 등 다양한 형광체 및 등급으로 제공됩니다.

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II molecules and is crucial for T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse thymocytes and splenocytes
  • Recommended ≤ 0.125 µg/test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper cells and also found in monocytes, macrophages, and dendritic cells. At the tissue level, CD4 is detected in thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut. CD4 facilitates early T-cell activation via association with Lck kinase and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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