Thermo Fisher Scientific Phospho-Zap-70 (Tyr319, Tyr352) Monoclonal Antibody (GT364)
인간 ZAP-70/Syk 단백질의 인산화 Tyr319, Tyr352 잔기를 인식하는 단클론 항체. Western blot에 적합하며, T세포 신호 전달 연구에 활용 가능. 마우스 IgG2a 형식, Protein A 정제, 액상 형태로 제공.
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Thermo Fisher Scientific Phospho-Zap-70 (Tyr319, Tyr352) Monoclonal Antibody (GT364)
Applications
- Western Blot (WB): 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT364 |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human ZAP70 (phospho Tyr319)/Syk (phospho Tyr352). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866542 |
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and plays a crucial role in T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319. Phosphorylation at Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while Tyr292 phosphorylation terminates activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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