Thermo Fisher Scientific BCL10 Recombinant Rabbit Monoclonal Antibody (BL10, 2988R)
BCL10 단백질을 표적하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. 인간 시료에 반응하며 IHC, ICC/IF, Flow Cytometry에 사용 가능. 고순도 Protein A/G 정제, 200 µg/mL 농도, PBS 기반 보존액 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BL10, 2988R |
| Immunogen | Recombinant human BCL10 protein fragment (around aa122–168) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
BCL-10 is an N-terminal CARD (Caspase Recruitment Domain) containing protein involved in the adaptive immune response. It promotes apoptosis, pro-caspase-9 maturation, and activation of NF-kappa-B via NIK and IKK.
BCL-10 acts as an adapter between the upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex and is a substrate for MALT1.
Mutations in the BCL10 gene can lead to Immunodeficiency 37 or mucosa-associated lymphoid type lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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