Thermo Fisher Scientific Cyclin B1 (G2- and M-phase Cyclin) Monoclonal Antibody (SPM619)
Cyclin B1 단백질을 인식하는 mouse monoclonal antibody (Clone SPM619). IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. G2/M 세포주기 조절 연구에 적합하며, 인간 및 마우스 시료에 반응. 단백질 A/G 정제, 액상 형태로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM619 |
| Immunogen | Recombinant human full-length CCNB1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs).
Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition.
It complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been identified: one constitutively expressed and another cell cycle–regulated, expressed mainly during the G2/M phase.
Cyclin B1 accumulates during G2 phase and undergoes APC-dependent degradation at the end of mitosis, a process required for proper cell cycle progression.
Overexpression of Cyclin B1 has been reported in several cancers, including breast, prostate, and non–small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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