Thermo Fisher Scientific Cyclin B1 (G2- and M-phase Cyclin) Monoclonal Antibody (SPM619)
Cyclin B1 단백질을 인식하는 단클론 항체로, G2/M기 세포주기 조절 연구에 적합. IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. Human 및 Mouse 시료에 반응하며, Protein A/G로 정제된 액상 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM619 |
| Immunogen | Recombinant human full-length CCNB1 protein (Cyclin B1, UniProt ID: P14635-1, antigen range: 1–433 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein essential for controlling the cell cycle at the G2/M transition. It complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts exist — one constitutively expressed and another cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is degraded at the end of mitosis through APC-dependent mechanisms. It is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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