Thermo Fisher Scientific F(Ab`)2 Biotin Polyclonal Antibody, Cyanine3
Goat-derived polyclonal F(ab')2 antibody conjugated with Cyanine3 for ICC/IF and flow cytometry. High specificity to biotin-KLH immunogen. Lyophilized form with stable storage at 4°C or -20°C. Excitation/emission at 554/566 nm for bright fluorescence.
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Applications
Immunocytochemistry (ICC/IF)
- Tested dilution: 1:100–1:1,000
Flow Cytometry (Flow)
- Tested dilution: 1:200–1:400
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Cyanine3 |
| Excitation/Emission Max | 554 / 566 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotin conjugated Goat IgG and biotin conjugated BSA. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7). It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is necessary for incorporation of carbon dioxide into organic compounds and exhibits a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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