Thermo Fisher Scientific Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, FITC

Thermo Fisher Scientific Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, FITC

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• AP
• APC
• Biotin
• HRP
• PE
• TRITC
• Custom conjugation available upon request

Product Specific Information

• Concentration may vary slightly between lots; refer to lot-specific datasheet.
• Product #31630 has been validated for Western blot, IF, ICC, IHC, IP, and FACS applications.
• Reacts with heavy chains of mouse IgG subclasses 1, 2a, 2b, and 3, but not with light chains or mouse IgM.
• Tested by ELISA and/or solid-phase adsorption to minimize cross-reactivity with human, bovine, and rabbit serum proteins.
• Store protected from light at 4°C. For extended storage, add glycerol to reach 50% and store at -20°C.
• Fluorescein Amax = 492 nm; Emax = 520 nm.
• Fluorophore/Protein ratio: 7.7 µg/mg (~3 moles FITC per mole IgG, lot-dependent).
• Reconstitute with 0.75 mL of distilled water before use.

Target Information

Anti-Mouse secondary antibodies are affinity-purified and provide specific detection of mouse immunoglobulins. They are widely used for detection, sorting, or purification purposes. Secondary antibodies enhance versatility and sensitivity through multiple detection systems (HRP, AP, fluorescence) and signal amplification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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