Thermo Fisher Scientific Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, FITC
Thermo Fisher Scientific의 Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, FITC는 마우스 IgG Fc를 특이적으로 인식하는 형광 표지 2차 항체로, IHC, ICC/IF, Flow Cytometry 등 다양한 면역 분석에 적합합니다. 높은 순도와 낮은 교차 반응성을 갖추었으며, FITC 형광(498/517 nm)을 통해 안정적인 신호 검출이 가능합니다.
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Thermo Fisher Scientific Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, FITC
Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Immunohistochemistry (IHC) | 1:50 - 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 - 1:200 |
| Flow Cytometry (Flow) | 1:50 - 1:200 |
| Immunoprecipitation (IP) | 1:50 - 1:200 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Lyophilized |
| Concentration | 1.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_429705 |
| Target | IgG Fc |
| Cross Adsorption | Against human, bovine, and rabbit serum proteins |
| Antibody Form | Whole Antibody |
| Country of Origin | USA |
Available Conjugate Formats
- AP
- APC
- Biotin
- HRP
- PE
- TRITC
- Custom conjugation available upon request
Product Specific Information
- Concentration may vary slightly between lots; refer to lot-specific datasheet.
- Product #31630 has been validated for Western blot, IF, ICC, IHC, IP, and FACS applications.
- Reacts with heavy chains of mouse IgG subclasses 1, 2a, 2b, and 3, but not with light chains or mouse IgM.
- Tested by ELISA and/or solid-phase adsorption to minimize cross-reactivity with human, bovine, and rabbit serum proteins.
- Store protected from light at 4°C. For extended storage, add glycerol to reach 50% and store at -20°C.
- Fluorescein Amax = 492 nm; Emax = 520 nm.
- Fluorophore/Protein ratio: 7.7 µg/mg (~3 moles FITC per mole IgG, lot-dependent).
- Reconstitute with 0.75 mL of distilled water before use.
Target Information
Anti-Mouse secondary antibodies are affinity-purified and provide specific detection of mouse immunoglobulins. They are widely used for detection, sorting, or purification purposes. Secondary antibodies enhance versatility and sensitivity through multiple detection systems (HRP, AP, fluorescence) and signal amplification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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