Thermo Fisher Scientific Pro-Detect Rapid Human Fc Assay Kit

Thermo Fisher Scientific Pro-Detect Rapid Human Fc Assay Kit

The Thermo Scientific™ Pro-Detect™ Rapid Human Fc Assay Kit is a single-use, dipstick lateral flow kit for quick and easy detection of human Fc-tagged proteins in protein expression and purification systems.
This assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the human Fc tag, providing a visual color readout within ten minutes.
It enables detection directly from cell culture media (supernatant) or cell lysates without any special instrumentation.

주요 특징

• Easy to adopt — Lateral flow strips can be used early and throughout expression to monitor and confirm expression of desired Fc-tagged proteins
• Simple, fast method — Dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5–10 minutes
• Sensitive — Detects protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
• Specific — No cross-reactivity with common lysis and protein preparation reagents
• Reliable — Comparable results to western blot and ELISA
• Long shelf life — Stable for at least one year at 4°C

작동 원리

Each Pro-Detect Rapid assay is performed by adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip.
Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample.
These complexes migrate along the strip membrane and bind at the test and control lines, displaying results as red bands.

사양