Thermo Fisher Scientific Pro-Detect Rapid GST Assay Kit

Thermo Fisher Scientific Pro-Detect Rapid GST Assay Kit

The Thermo Scientific™ Pro-Detect™ Rapid GST Assay Kit is a single-use, dipstick lateral flow kit for quick and easy detection of glutathione S transferase (GST)-tagged proteins in protein expression and purification systems.
This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the GST tag to provide a visual, color readout for detection within ten minutes.
It can detect GST-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features

• Easy to adopt: Lateral flow strips can be used early and throughout expression to monitor and confirm expression of the desired protein-tagged target
• Simple, fast method: Dilute sample using provided diluent and dip the lateral flow strip for visual results within 5–10 minutes
• Sensitive: Detects protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
• Specific: Assay does not cross-react with common lysis and protein preparation reagents
• Reliable: Results comparable to other antibody immunoassays such as western blot and ELISA
• Long shelf life: Stable for at least one year at 4°C

Assay Principle

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip.
Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes migrate along the strip membrane and bind at the test and control lines, producing visible red bands.

Specifications