Thermo Fisher Scientific Pro-Detect Rapid MBP Assay Kit

Thermo Fisher Scientific Pro-Detect Rapid MBP Assay Kit

The Thermo Scientific™ Pro-Detect™ Rapid MBP Assay Kit is a single-use, dipstick lateral flow kit for quick and easy detection of maltose binding protein (MBP)-tagged proteins in protein expression and purification systems.
This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the MBP tag to provide a visual, color readout for detection within ten minutes.
The assay can detect MBP-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features

• Easy to adopt — lateral flow strips can be used early and throughout expression to monitor and confirm expression of desired MBP-tagged targets
• Simple, fast method — dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5–10 minutes
• Sensitive — detect protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
• Specific — no cross-reactivity with common lysis and protein preparation reagents
• Reliable — results comparable to western blot and ELISA
• Long shelf life — stable for at least one year at 4°C

Assay Principle

Each Pro-Detect Rapid assay is performed by adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip.
Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample.
These complexes travel along the strip membrane and bind at the test and control lines, displaying red bands as visual results.

Specifications

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