Thermo Fisher Scientific Phospho-Syk (Tyr525, Tyr526) Recombinant Rabbit Monoclonal Antibody (PSH03-70)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH03-70

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr525 and 526 of Human SYK. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Syk,uniProtId:P43405-1,ncbiNodeId:9606,antigenRange:525|526,antigenLength:635,antigenImageFileName:MA5-56382_Syk_P43405-1_Rabbit.svg,antigenImageFileNamePDP:MA5-56382_Syk_P43405-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive Control: Ramos serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, Ramos cells serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes.

Tissue Specificity: Tissue enhanced (lymphoid tissue, parathyroid gland).

Subcellular Location: Cell membrane, Cytoplasm, cytosol.

Sequence Similarities: 100% Mouse/Rat.

Predicted band size: 72 kDa.

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.