Thermo Fisher Scientific H-Ras Recombinant Rabbit Monoclonal Antibody (4Q7M5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4Q7M5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 90-189 of human HRAS (P01112) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H-Ras,uniProtId:P01112-1,ncbiNodeId:9606,antigenRange:90-189,antigenLength:189,antigenImageFileName:MA5-35323_H-Ras_P01112-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35323_H-Ras_P01112-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849225

Product Specific Information

Immunogen sequence: FEDIHQYREQ IKRVKDSDDV PMVLVGNKCD LAARTVESRQ AQDLARSYGI PYIETSAKTR QGVEDAFYTL VREIRQHKLR KLNPPDESGP GCMSCKCVLS

Target Information

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K-, N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibition of apoptosis. K-Ras mutations are common in pancreatic, colorectal and nonsmall-cell lung carcinomas; H-Ras mutations are common in bladder, kidney and thyroid carcinomas; N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinaoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.