Thermo Fisher Scientific H-Ras Recombinant Rabbit Monoclonal Antibody (4Q7M5)
H-Ras 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 친화 크로마토그래피 정제. -20°C 보관, 연구용 전용 시약.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4Q7M5 |
| Immunogen | Synthetic peptide corresponding to amino acids 90–189 of human HRAS (P01112) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849225 |
Product Specific Information
Immunogen sequence:
FEDIHQYREQ IKRVKDSDDV PMVLVGNKCD LAARTVESRQ AQDLARSYGI PYIETSAKTR QGVEDAFYTL VREIRQHKLR KLNPPDESGP GCMSCKCVLS
Target Information
Monomeric G protein Ras acts as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Mammalian cells contain three Ras proto-oncogenes coding for closely related proteins: H-, K-, and N-Ras. Oncogenic mutations in Ras genes are found in approximately 30% of human cancers. Constitutive activation of Ras due to mutation or overexpression promotes proliferation and inhibits apoptosis.
- K-Ras mutations: pancreatic, colorectal, non-small-cell lung carcinomas
- H-Ras mutations: bladder, kidney, thyroid carcinomas
- N-Ras mutations: melanoma, hematological malignancies, hepatocellular carcinoma
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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