Thermo Fisher Scientific Thioredoxin 2 Polyclonal Antibody
Thioredoxin 2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 고순도 Protein A 정제 및 안정적인 저장용액 구성으로 연구 재현성이 우수함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Thioredoxin 2, amino acids 101–166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor.
Reduced Trx acts as a hydrogen donor for ribonucleotide reductase, an essential enzyme for DNA synthesis, and serves as a general protein disulfide reductase involved in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and transcription factors such as p53, NF-κB, and AP-1.
Trx also removes H₂O₂ when coupled with methionine sulfoxide reductase or various peroxiredoxin isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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