Thermo Fisher Scientific AKT1 Recombinant Rabbit Monoclonal Antibody (4B6)
AKT1 단백질을 인식하는 Recombinant Rabbit Monoclonal Antibody (Clone 4B6). Western blot, IHC, ICC/IF 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, 세포 생존 및 신호전달 연구에 적합. 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4B6 |
| Immunogen | A synthesized peptide derived from human AKT1 (UniProt ID: P31749-1, antigen range: 400–480) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.28 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898304 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1, and other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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