Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PerCP-Cyanine5.5, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), PerCP-Cyanine5.5, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.06 µg)/test
• Tested for intracellular staining of stimulated human peripheral blood cells
• Recommended for use with Blue Laser (Excitation 488 nm, Emission 695 nm)

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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 650
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine7, PE-eFluor 610
• Custom conjugation available

Product Specific Information

The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFNγ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic activity. It synergizes with TNFα to inhibit proliferation and modulate immune responses across various cell types. Glycosylation does not affect biological activity.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-titrated and validated on stimulated human peripheral blood cells
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFNγ (Interferon gamma, Type II interferon) is a macrophage activation factor produced by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) with species-specific activity—human IFNγ does not cross-react with mouse.

Functions of IFNγ:

• Antiviral and tumor antiproliferative activity
• Induction of MHC class I and II expression
• Macrophage activation
• Enhanced immunoglobulin secretion by B cells
• Cytokine regulation via JAK-STAT pathway activation

Human IFNγ shares ~40% sequence homology with mouse IFNγ. Expression is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFNγ gene are associated with aplastic anemia.

For Research Use Only.
Not for diagnostic procedures or resale without authorization.

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