Thermo Fisher Scientific JAK3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-530 of JAK3 (NP_0002062) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JAK3,uniProtId:P52333-1,ncbiNodeId:9606,antigenRange:350-530,antigenLength:1124,antigenImageFileName:PA5-87500_JAK3_P52333-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87500_JAK3_P52333-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.13 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804203

Product Specific Information

Immunogen sequence: RLTTDSQHFF CKEVAPPRLL EEVAEQCHGP ITLDFAINKL KTGGSRPGSY VLRRSPQDFD SFLLTVCVQN PLGPDYKGCL IRRSPTGTFL LVGLSRPHSS LRELLATCWD GGLHVDGVAV TLTSCCIPRP KEKSNLIVVQ RGHSPPTSSL VQPQSQYQLS QMTFHKIPAD SLEWHENLGH G

Positive Samples: U-87MG; Cellular Location: Cytoplasm, Endomembrane system, Peripheral membrane protein

Target Information

Janus kinase 3 (JAK3) phosphorylates members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to activation of interleukin receptors. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. However, active JAK1 may be required for complex responses. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.