Thermo Fisher Scientific GUCY1B3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total GCYB1.

Target Information

Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family.
There are two forms of guanylate cyclase: a soluble form (GCS or sGC), which acts as a receptor for nitric oxide, and a membrane-bound receptor form (GC), which serves as a peptide hormone receptor.
The GC-C protein is composed of:

• An extracellular domain
• A single transmembrane domain
• A cytoplasmic region consisting of a kinase-like domain and a catalytic domain

It is expressed as two differentially glycosylated forms:

• A 130 kDa precursor form present in the endoplasmic reticulum
• A 145 kDa form present on the plasma membrane

Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP.
GC-C acts as the receptor for heat-stable enterotoxins, small peptides secreted by pathogenic strains of E. coli that cause severe secretory diarrhea.
GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.