Thermo Fisher Scientific SMAD1/SMAD5/SMAD9 Polyclonal Antibody
SMAD1/SMAD5/SMAD9 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성. 고순도 Affinity chromatography 정제, 1 mg/mL 농도. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMAD1 (Accession Q15797), corresponding to amino acid residues I438–S465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2853824 |
Product Specific Information
Antibody detects endogenous levels of total Smad1/5/9.
Target Information
The protein encoded by SMAD1/SMAD5/SMAD8/SMAD9 belongs to the SMAD family, similar to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
This protein mediates the signals of bone morphogenetic proteins (BMPs), which regulate cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand binding, it becomes phosphorylated and forms a complex with SMAD4, crucial for transcriptional regulation.
It is a target for SMAD-specific E3 ubiquitin ligases such as SMURF1 and SMURF2, undergoing ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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