Thermo Fisher Scientific ATP5I Monoclonal Antibody (OTI1E6), TrueMAB
ATP5I 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 검증 완료. 인간 시료에 반응하며, E.coli에서 생산된 ATP5I 전장 단백질을 면역원으로 사용. 동결건조 형태로 제공되며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E6 |
| Immunogen | Full-length human recombinant ATP5I protein produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve an antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Mitochondrial ATP synthases (ATPases) convert membrane electrochemical proton gradients into energy for synthesizing high-energy phosphate bonds.
They consist of two linked complexes:
- F1: Hydrophilic catalytic core
- F0: Membrane-embedded protein channel
ATP5I (also known as mitochondrial ATP synthase subunit E or ATP5K) is a 69 amino acid protein localized to the inner mitochondrial membrane and part of the F0 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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