Thermo Fisher Scientific SLC12A1 Polyclonal Antibody, Biotin
SLC12A1(NKCC2) 단백질을 인식하는 Biotin 결합 폴리클로날 항체로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용 가능. Rabbit IgG 기반으로 고순도 친화 크로마토그래피 정제. 인체, 마우스, 원숭이, 랫트 반응성. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 30–60 on NKCC2 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The sodium-potassium-chloride cotransporter isoform 2 (NKCC2) is kidney-specific and localized on the apical membrane of the thick ascending limb of Henle’s loop and the macula densa. It mediates NaCl resorption with a stoichiometry of 1Na:1K:2Cl and is sensitive to diuretics such as furosemide and bumetanide. Mutations in this gene can cause Bartter-like syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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