Thermo Fisher Scientific Placental Alkaline Phosphatase Polyclonal Antibody
인간 PLAP 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 염증 조절 및 생식세포 종양 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 2–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to human ALPP (403–433 aa, sequence: KARDRKAYTVLLYGNGPGYVLKDGARPDVTE) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2745880 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a final concentration of 500 µg/mL.
Target Information
Placental Alkaline Phosphatase (PLAP) plays an important role in regulating inflammatory processes. There are four related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney types. PLAP is a membrane-bound isoenzyme (Regan and Nagao type) expressed in the placenta during the third trimester of gestation.
PLAP is a useful marker for identifying testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
A proposed function of PLAP is matrix mineralization; however, mice lacking functional PLAP show normal skeletal development. PLAP has been directly linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects. The severity and onset of hypophosphatasia vary depending on the specific mutation involved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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