Thermo Fisher Scientific CD45 Polyclonal Antibody
Rabbit 폴리클로날 항체로 다양한 응용(WB, IHC, ICC, IP)에 사용 가능. 인간 CD45 단백질의 1254–1304 아미노산 구간을 면역원으로 제작. 액상 형태, 1 mg/mL 농도, 4°C 보관. 림프구 발달 및 항원 신호 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1254 to 1304 of human CD45 molecule |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC: Acetone or formaldehyde fixation recommended.
- Permeabilization with Triton X-100 is recommended for formaldehyde-fixed cells.
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, comprising about 10% of all surface proteins in lymphocytes. It is absent in non-hematopoietic tissues and cell lines.
CD45 plays a critical role in lymphocyte development and antigen signaling by regulating Src-family kinases. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains.
CD45RA, one isoform, shows restricted expression among lymphoid cell subtypes. CD45 is essential for T- and B-cell receptor signaling and modulates cytokine receptor pathways through suppression of JAK kinases. It also influences cell survival, apoptosis, and integrin-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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