Thermo Fisher Scientific IFN gamma Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Rat IFN-gamma. Anti-Rat IFN-gamma-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat IFN-gamma by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IFN-gamma (500-P122G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IFN-gamma.

Western Blot:
To detect Rat IFN-gamma by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Rat IFN-gamma is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P122GBT-1MG will be provided as 2 × 500 µg.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein of two 146 amino acid subunits (~45 kDa as a homodimer). On SDS-PAGE, it appears as 25, 20, and 15.5 kDa bands due to differential glycosylation.

Functions:

• Antiviral and tumor antiproliferative activity
• Induction of class I and II MHC
• Macrophage activation
• Enhancement of immunoglobulin secretion by B lymphocytes
• Cytokine regulation and synergistic activity with other cytokines

Mechanism:
Activation occurs through binding to IFN gamma receptor I and II, triggering the JAK-STAT pathway.
Human IFN gamma shows ~40% sequence homology with mouse IFN gamma but no cross-reactivity.

Regulation:
Upregulated by IL-2, FGF basic, and EGF; downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.