Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PerCP-eFluor 710, eBioscience
Mouse CD8β 인식 Rat IgG2b 단클론 항체로 PerCP-eFluor 710 형광 표지. Flow cytometry에 최적화되어 있으며 0.06 µg/test 권장. CD8α/β T 세포 분석에 적합하며 488 nm Blue laser에서 710 nm 방출. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PerCP-eFluor 710
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.06 µg/test
- Publications: View 13 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH35-17.2 (H35-17.2) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669709 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule, which associates with CD8 alpha to form the CD8α/β heterodimer expressed on thymocytes and cytotoxic T cells. CD8 binds MHC class I and plays a role in T cell development and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.06 µg/test
- Recommended Use: Titrate for optimal performance
- Fluorochrome Info: PerCP-eFluor® 710 emits at 710 nm, excited by 488 nm blue laser
- Recommended filter: 710/50 bandpass (695/40 acceptable alternative)
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness or compensation.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes, mediating interactions within the immune system. It acts as a coreceptor with the T-cell receptor to recognize antigens presented by MHC class I molecules. The functional receptor is either a homodimer (α/α) or heterodimer (α/β). Both chains share homology to immunoglobulin variable light chains.
This gene encodes CD8 beta chain isoforms, with multiple splice variants and a pseudogene located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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