Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PerCP-eFluor 710

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.06 µg/test
• Publications: View 13 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule, which associates with CD8 alpha to form the CD8α/β heterodimer expressed on thymocytes and cytotoxic T cells. CD8 binds MHC class I and plays a role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.06 µg/test
• Recommended Use: Titrate for optimal performance
• Fluorochrome Info: PerCP-eFluor® 710 emits at 710 nm, excited by 488 nm blue laser
  • Recommended filter: 710/50 bandpass (695/40 acceptable alternative)

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness or compensation.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes, mediating interactions within the immune system. It acts as a coreceptor with the T-cell receptor to recognize antigens presented by MHC class I molecules. The functional receptor is either a homodimer (α/α) or heterodimer (α/β). Both chains share homology to immunoglobulin variable light chains.
This gene encodes CD8 beta chain isoforms, with multiple splice variants and a pseudogene located on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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