Thermo Scientific™

DH5α Competent Cells for Subcloning

Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors.자세히 알아보기

Thermo Scientific DH5α Competent Cells for Subcloning are chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

• Transformation efficiency: >1x106 cfu/μg pUC19 DNA
• Suitable for routine cloning applications
• Convenient economical packaging
• Contain genetic markers that allow for blue/white colony screening

Applications
DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
• DNA cloning following PCR or restriction digestion reactions
• Subcloning (transferring DNA from a parent vector to a destination vector)
• Plasmid isolation

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

사양

제품 유형Competent Cell

에프에피솜 포함Lacks F` Episome

효율1x10^6 cfu/μg

세포 유형Chemically Competent

메틸화 DNA 클로닝No

박테리아 또는 효모 균주DH5α

블루/화이트 스크리닝Yes

고처리량 호환성Not High-throughput Compatible

ccdB 벡터 증식Not for ccdB vector propagation

배송 조건Dry Ice

종E. coli

형식Tube

제품라인Thermo Scientific™

용도(애플리케이션)Cloning

수량4 x 500 μL

Unit SizeEach