Thermo Scientific™

DH5α Competent Cells

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or자세히 알아보기

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.

• High transformation efficiency: >1x109 cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesis

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

사양

제품 유형Competent Cell

에프에피솜 포함Lacks F` Episome

효율High Efficiency (>1x10^9 cfu/μg pUC19 DNA)

세포 유형Chemically Competent

라이브러리cDNA

메틸화 DNA 클로닝No

박테리아 또는 효모 균주DH5α

블루/화이트 스크리닝Yes

고처리량 호환성Not High-throughput Compatible

ccdB 벡터 증식Not for ccdB vector propagation

배송 조건Dry Ice

종E. coli

형식Tube

제품라인Thermo Scientific™

용도(애플리케이션)Cloning

수량10 x 100 μL

Unit SizeEach