Thermo Scientific™

DH10B Competent Cells

Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning자세히 알아보기

Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently. These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.

• High transformation efficiency: >1x109 cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Allows for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH10B Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning both of prokaryotic and eukaryotic origin
• Cloning of large plasmids and BACs
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site directed mutagenesis

Genotype
F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80_lacZ_ΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG

Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542).

사양

제품 유형Competent Cell

에프에피솜 포함Lacks F` Episome

효율High Efficiency (>1x10^9 cfu/μg pUC19 DNA)

세포 유형Chemically Competent

라이브러리cDNA

메틸화 DNA 클로닝Yes

박테리아 또는 효모 균주DH10B™

블루/화이트 스크리닝Yes

고처리량 호환성Not High-throughput Compatible

ccdB 벡터 증식Not for ccdB vector propagation

배송 조건Dry Ice

종E. coli

형식Tube

제품라인Thermo Scientific™

용도(애플리케이션)Cloning

수량10 x 100 μL

Unit SizeEach