Thermo Fisher Scientific Eif3s6 Int6 Polyclonal Antibody
Rabbit polyclonal antibody targeting eIF3S6/Int6 protein. Reactive with multiple species including human, mouse, and rat. Validated for Western blot and ELISA. Supplied as liquid, affinity purified, and stored at -20°C for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:90,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid repeated freeze-thaw cycles. Centrifuge the product if not completely clear after standing at room temperature. The product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis suggests cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.
Target Information
The eukaryotic translation initiation factor 3 (eIF-3) complex is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Additionally, it is required for nonsense-mediated mRNA decay (NMD) and may act with UPF2 to divert mRNAs from translation to the NMD pathway. It may interact with MCM7 and EPAS1 and regulate proteasome-mediated degradation of these proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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