Thermo Fisher Scientific CEBP delta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:15,000

-

Immunohistochemistry (IHC)

1:2,000

-

ELISA (ELISA)

1:4,000-1:9,000

-

Immunoprecipitation (IP)

0.8-1.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:C/EBP delta,uniProtId:Q00322-1,ncbiNodeId:10090,antigenRange:1,antigenLength:268,antigenImageFileName:600-401-A61_CEBP_delta_Q00322-1_Rabbit.svg,antigenImageFileNamePDP:600-401-A61_CEBP_delta_Q00322-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.

Target Information

C/EBP delta are CCAAT enhancer binding proteins (CEBPs), a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. At least six members of the family have been isolated and characterized to date (CEBP alpha to CEBP zeta). CEBPD is a leucine zipper (LZ) DNA-binding protein that regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. CEBPD is an important transcriptional activator in the regulation of genes involved in immune and inflammatory responses and has been reported to possess many tumor suppressor-like properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.