Thermo Fisher Scientific DNAJA2 Monoclonal Antibody (OTI3A1), TrueMAB
DNAJA2 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. HEK293T 세포에서 생산된 full-length recombinant protein을 면역원으로 사용. 연구용으로 단백질 접힘 및 Hsp70 보조단백질 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A1 |
| Immunogen | Full length human recombinant protein of human DNAJA2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The DNAJA2 protein belongs to the evolutionarily conserved DNAJ/HSP40 family, which regulates molecular chaperone activity by stimulating ATPase activity.
DNAJ proteins may have up to three distinct domains:
- A conserved 70-amino acid J domain (usually at the N terminus)
- A glycine/phenylalanine-rich region
- A cysteine-rich domain containing four motifs resembling a zinc finger domain
DNAJA2 acts as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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