Thermo Fisher Scientific CD43 Recombinant Rabbit Monoclonal Antibody (SPN, 1766R)
CD43 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 인간 시료 반응성이 있으며 HEK293 발현 시스템에서 생산되었습니다. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태로 제공됩니다.
- 카탈로그번호
- 6693-RBM6-P1ABX
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SPN, 1766R |
| Immunogen | Recombinant full-length human SPN protein (CD43, UniProt ID: P16150-1, range 1–400) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, then cool at room temperature for 20 min.
Target Information
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. It contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation. Through interaction with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte traffic.
CD43 signaling enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. Its expression induces tumor suppressor p53 protein; however, in p53 and ARF deficiency, CD43 promotes tumor proliferation.
CD43 dysfunction is associated with Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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