Thermo Fisher Scientific CD43 Recombinant Rabbit Monoclonal Antibody (SPN, 1766R)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, then cool at room temperature for 20 min.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. It contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation. Through interaction with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte traffic.
CD43 signaling enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. Its expression induces tumor suppressor p53 protein; however, in p53 and ARF deficiency, CD43 promotes tumor proliferation.
CD43 dysfunction is associated with Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.